Interview Questions on HPLC

Interview Questions on HPLC

  1. Which of the following is not true about High pressure liquid chromatography (HPLC)?

a) It requires high pressure for the separation of the specious
b) There is no need to vaporise the samples
c) It is performed in columns
d) It has high sensitivity

Answer: b

Explanation: In High pressure liquid chromatography (HPLC), samples need to be vaporised. It has high sensitivity.

  1. High pressure liquid chromatography can be performed only in columns.

a) True
b) False

Answer: a

Explanation: High pressure liquid chromatography can be performed only in columns. This is because application of high pressure in open bed will not be effective.

  1. Which of the following is not an advantage of Syringe type pumps used in High pressure liquid chromatography?

a) Independent of viscosity
b) Pulse-less flow
c) High pressure capability
d) Unlimited solvent capacity

Answer: d

Explanation: The limitation of Syringe type pump is that it has limited solvent capacity and is inconvenient when solvents are to be changed.

  1. Which of the following is not true about solvent programming which is done in high performance liquid chromatography?

a) It provides unequal bandwidths
b) It provides fast overall separation
c) It provides maximum resolution
d) It provides maximum sensitivity

Answer: a

Explanation: Solvent programming is done to provide equal bandwidths. It provides maximum sensitivity for every solute in the sample.

  1. Which of the following pulse damper takes up some amount of the pulsation energy which is released to provide smooth pressure without pulsations?

a) Flexible bellows or compressible gas passed through tee columns
b) Flexible inert diaphragm
c) Electronic pulse damper
d) Electrical pulse damper

Answer: a

Explanation: Flexible bellows or compressible gas passed through tee columns take up some of the pulsation energy which is released to provide smooth pressure without pulsations. Its main purpose is to avoid pulses.

  1. Which of the following is not a characteristic of the syringe pump used in high pressure liquid chromatography?

a) Pressure capability is high
b) Maintenance is frequent
c) Limited reservoir capability
d) Slight change of flow rate when extremely high pressure compresses the solvent

Answer: b

Explanation: In syringe pumps, maintenance is infrequent. However, it has high pressure capability.

  1. Syringe pumps used in High pressure liquid chromatography are most suitable for which of the following columns?

a) Capillary columns
b) Guard columns
c) Short-fast columns
d) Small bore columns

Answer: d

Explanation: Syringe pumps used in High pressure liquid chromatography are most suitable for small bore columns. It is the most commonly used piston type pump.

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